The results indicated that human uterine leiomyomal cells express bcl-2 protein and progesterone enhances its expression, however, sb reduces the expression of bcl-2 protein in human uterine leiomyoma cells. Uterine leiomyoma is the most common benign smooth muscle cell tumor of the myometrium although bcl-2 protein is known to be an apoptosis-inhibiting gene product and to prevent apoptotic cell death in a variety of cells, there are no published data regarding whether human leiomyomas express bcl-2 protein. Cd mimics the effects of estrogen in the rat uterus, and blood cd concentrations positively correlate with er levels in uteri of women with fibroids in the present study we explored whether cd could stimulate proliferation of estrogen-responsive human uterine leiomyoma (ht-utlm) cells and uterine smooth muscle cells (ht-utsmcs) through.
Background: statins have broad reaching effects beyond lowering plasma lipids including anti-tumor properties results: simvastatin inhibits proliferation and induces calcium-dependent apoptosis of human uterine leiomyoma cells conclusion: we report a novel calcium-mediated pathway associated with anti-tumor properties of simvastatin significance: simvastatin may have anti-tumor properties. Background uterine leiomyomas (fibroids) are benign smooth muscle tumors that often contain an excessive extracellular matrix (ecm) in the present study, we investigated the interactions between human uterine leiomyoma (utlm) cells and uterine leiomyoma-derived fibroblasts (fb), and their importance in cell growth and ecm protein production using a coculture system. Because uterine leiomyomas are characterized by excessive ecm production, we evaluated the effect of dehp on type i collagen expression by western blot analyses in human myometrial cells (n = 6) and leiomyoma cells (n = 6. Objective uterine leiomyomas are the most common gynecological benign tumor and greatly affect reproductive health and well-being they are the predominant indication for hysterectomy in premenopausal women.
Title = effects of adlay hull and testa ethanolic extracts on the growth of uterine leiomyoma cells, abstract = uterine leiomyoma has become a significant health concern affecting one in forth women in reproductive age in taiwan, according to the ministry of health and welfare. The immortalized human uterine leiomyoma cell line used in these studies represents a system superior to primary cells, which senesce early, to study the molecular character. The proliferation rate of uterine leiomyoma cells was determined the apoptosis rate of uterine leiomyoma cells was determined the phosphorylation levels of egfr from uterine leiomyoma cells were measured objective: to explore the effect of euonine on the proliferation and apoptosis of uterine. We previously demonstrated that berberine (bbr) inhibits cell proliferation and induces apoptosis in a human uterine leiomyoma (utlm) cell line but does not demonstrate a significant cytotoxic effect in a normal human uterine smooth muscle (utsm) cell line. Effect of different ingredients in traditional korean medicine for human uterine leiomyoma on normal myometrial and leiomyomal smooth muscle cell proliferation prati bajracharya1,, eun ju lee1,, dong-mok lee2, sang hee shim1,2,.
Uterine leiomyoma is the most common benign tumor type of the female reproductive tract despite its high prevalence, the exact pathogenesis of the benign tumor remains unknown in the present study, the effects of di‑(2‑ethylhexyl) phthalate (dehp) on the proliferation and apoptosis rates and. The purpose of this study was to examine the anti-proliferative effect of 2-me in vitro and in vivo, in a mouse xenograft model using human leiomyosarcoma sk-lms-1 cell line method : to performed in vitro experiment, we evaluated anti-proliferative effect of 2-me on sk-lms-1 cells using mtt assay and western blot. Effect of euonine on the proliferation and apoptosis of human uterine leiomyoma cells running title: role of euonine on uterine leiomyoma highlights: the proliferation rate of uterine leiomyoma cells was determined the apoptosis rate of uterine leiomyoma cells was determined. Background: uterine leiomyoma is the most common benign tumor in reproductive-age women each leiomyoma is thought to be a benign monoclonal tumor arising from a single transformed myometrial. Objective: to evaluate the effects of selective p receptor (pr) modulator cdb4124 on cell proliferation and apoptosis in cultured human uterine leiomyoma smooth muscle (lsm) cells and control myometrial smooth muscle (msm) cells in matched uteri.
Background: due to dietary exposure of women to genistein, a soy-derived phytoestrogen, and the estrogen responsiveness of uterine leiomyomas 'fibroids', we evaluated the effects of genistein (0001-50 microg/ml) on human uterine leiomyoma (utlm) cells versus uterine smooth muscle cells (utsmcs) in vitro. Two essential features of leiomyoma tumors, which arise from the uterine smooth muscle cells, are an increase in smooth muscle cell (smc) proliferation and excessive, aberrant deposition of extracellular matrix (ecm) collagen. Simvastatin potently stimulated leiomyoma cell apoptosis in a manner mechanistically dependent upon apoptotic calcium release from voltage-gated calcium channels therefore, simvastatin possesses antitumor effects that are dependent upon the apoptotic calcium release machinery. Introduction uterine leiomyoma (fibroids/myomas) is a common problem, occurring in 40% of women older than 35 years the availability of a safe and effective non-surgical treatment of leiomyoma would be of considerable clinical and public health importance. Kim: in 4973vitro effects of phthalate in human uterine leiomyoma cells the availability of hif-1 is determined primarily by hif-1α, which is regulated at the protein level in an oxygen-sensitive manner (20.
The cells were then used to test the effect of human recombinant tnf-α (sigma-aldrich) on the expression of mmp-2 and 9 protein and mrna tnf-α activates erk-dependent nf-κb pathway to up-regulate the expression of mmp-2 and stimulate cell migration in human uterine leiomyoma smcs the data are presented as the means with the 95%. It is believed that estrogen and progesterone have a mitogenic effect on leiomyoma cells and also act by influencing (directly and indirectly) a large number of growth factors, cytokines and apoptotic factors as well as other hormones. Objective to assess the effect of three wnt/β-catenin pathway inhibitors - inhibitor of β-catenin and tcf4 (icat), niclosamide, and xav939 - on the proliferation of primary cultures of human uterine leiomyoma cells. Uterine leiomyomas are benign smooth-muscle tumors with an estimated prevalence of 77% among women of reproductive age in the united states1 and can cause a range of health problems2 according to.
Antiproliferative effects by let-7 repression of high-mobility group a2 in uterine leiomyoma yi peng, jordan laser, guizhi shi, khush mittal, jonathan melamed, overexpression of hmga2 in human uterine leiomyomas is mainly contributed by its dominant transcript hmga2a by northern blot analysis (14) culture leiomyoma cells in the absence. The antifibrotic drug halofuginone inhibits proliferation and collagen production by human leiomyoma and myometrial smooth muscle cells [histological and biochemical studies of collagen in human uterine leiomyomas] s evelyn, fk lim, mh liu, el yonggrowth dynamics of human leiomyoma cells and inhibitory effects of the peroxisome.